Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography

Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography

Combining tomography with electron microscopy (EM) produces photos at definition adequate to visualise particular person protein molecules or molecular complexes in intact neurons. When freeze-substituted hippocampal cultures in plastic sections are imaged by EM tomography, detailed buildings rising from 3D reconstructions reveal putative glutamate receptors and membrane-associated filaments containing scaffolding proteins akin to postsynaptic density (PSD)-95 household proteins primarily …

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