Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes.
Transcription factor-based biosensors are helpful instruments for the detection of metabolites and industrially beneficial molecules, and current many potential purposes in biotechnology and biomedicine. However, the most typical strategy to develop biosensors depends on using a restricted set of naturally occurring allosteric transcription elements (aTFs). Therefore, altering the ligand specificity of aTFs in direction of the detection …