Human VIL1(Villin 1) ELISA Kit

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Villin 1 (VIL1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Villin 1 (VIL1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Villin 1 (VIL1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Villin 1 (VIL1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Villin 1 (VIL1) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Description: A competitive ELISA for quantitative measurement of Human Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Human Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Human Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Porcine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Porcine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Porcine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Canine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Canine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Canine Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Goat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Goat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Goat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Mouse Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Mouse Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Mouse Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rat Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Monkey Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Monkey Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Monkey Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A sandwich ELISA kit for detection of Villin 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: A sandwich ELISA kit for quantitative measurement of Human VIL1 (Villin 1) in samples from Serum, Plasma, Cell supernatant

Description: Quantitative sandwich ELISA for measuring Human Villin-1 (VIL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human Villin-1 (VIL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Quantitative sandwich ELISA for measuring Human Villin-1 (VIL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Description: Recombinant Human Villin 1 expressed in: E.coli

Description: A competitive ELISA for quantitative measurement of Guinea pig Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Guinea pig Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Guinea pig Villin 1(VIL1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A sandwich CLIA kit for quantitative measurement of Human VIL1 (Villin 1) in samples from Serum, Plasma, Cell supernatant

Description: A Monoclonal antibody against Human VIL1 / Villin. The antibodies are raised in Mouse. This antibody is applicable in IHC-P

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1)

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Human, Mouse, Pig Villin 1 (VIL1). This antibody is labeled with APC-Cy7.

Description: Enzyme-linked immunosorbent assay kit for quantification of Human Villin-1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: Rabbit Monoclonal Villin Antibody. Validated in WB and tested in Human, Mouse, Rat.

Description: Description of target: Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.13 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.7"Different calcium dependence of the capping and cutting activities of villin."_x005F_x005F_x000D_Northrop J., Weber A., Mooseker M.S., Franzini-Armstrong C., Bishop M.F., Dubyak G.R., Tucker M., Walsh T.P._x005F_x005F_x000D_J. Biol. Chem. 261:9274-9281(1986) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.8"Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton."_x005F_x005F_x000D_Zhai L., Zhao P., Panebra A., Guerrerio A.L., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 276:36163-36167(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, ASSOCIATION WITH F-ACTIN, PHOSPHORYLATION.Ref.11"Association of villin with phosphatidylinositol 4,5-bisphosphate regulates the actin cytoskeleton."_x005F_x005F_x000D_Kumar N., Zhao P., Tomar A., Galea C.A., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 279:3096-3110(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH PHOSPHATIDYLINOSITOL, MUTAGENESIS OF ARG-138; LYS-145; ARG-146; LYS-822 AND LYS-824.Ref.12"Identification of a functional switch for actin severing by cytoskeletal proteins."_x005F_x005F_x000D_Kumar N., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 279:24915-24918(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF ASP-467 AND ASP-715.Ref.13"Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin."_x005F_x005F_x000D_Kumar N., Tomar A., Parrill A.L., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 279:45036-45046(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CALCIUM-BINDING, MUTAGENESIS OF GLU-25; ASP-44; ASP-61; GLU-74; ASP-86 AND ALA-93.Ref.14"Regulation of cell motility by tyrosine phosphorylated villin."_x005F_x005F_x000D_Tomar A., Wang Y., Kumar N., George S., Ceacareanu B., Hassid A., Chapman K.E., Aryal A.M., Waters C.M., Khurana S._x005F_x005F_x000D_Mol. Biol. Cell 15:4807-4817(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF TYR-46; TYR-60; TYR-81 AND TYR-256, SUBCELLULAR LOCATION.Ref.15"Interaction of phospholipase C-gamma1 with villin regulates epithelial cell migration."_x005F_x005F_x000D_Tomar A., George S., Kansal P., Wang Y., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 281:31972-31986(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH PLCG1, PHOSPHORYLATION AT TYROSINE RESIDUES, MUTAGENESIS OF TYR-46; TYR-60; TYR-81; TYR-256; TYR-286; TYR-324; TYR-461; TYR-555; TYR-604 AND TYR-725, SUBCELLULAR LOCATION.Ref.16"Obligatory role for phospholipase C-gamma(1) in villin-induced epithelial cell migration."_x005F_x005F_x000D_Wang Y., Tomar A., George S.P., Khurana S._x005F_x005F_x000D_Am. J. Physiol. 292:C1775-C1786(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH PLCG1, PHOSPHORYLATION AT TYROSINE RESIDUES, MUTAGENESIS OF TYR-46; TYR-60; TYR-81; TYR-256; TYR-286; TYR-324; TYR-461; TYR-555; TYR-604 AND TYR-725, SUBCELLULAR LOCATION.Ref.17"Dimerization and actin-bundling properties of villin and its role in the assembly of epithelial cell brush borders."_x005F_x005F_x000D_George S.P., Wang Y., Mathew S., Srinivasan K., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 282:26528-26541(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, HOMODIMERIZATION, SUBCELLULAR LOCATION.Ref.18"Villin severing activity enhances actin-based motility in vivo."_x005F_x005F_x000D_Revenu C., Courtois M., Michelot A., Sykes C., Louvard D., Robine S._x005F_x005F_x000D_Mol. Biol. Cell 18:827-838(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF ASP-61; GLU-74; 86-ASP--GLY-91 AND 125-GLY--VAL-129, SUBCELLULAR LOCATION.Ref.19"Autotaxin and lysophosphatidic acid stimulate intestinal cell motility by redistribution of the actin modifying protein villin to the developing lamellipodia."_x005F_x005F_x000D_Khurana S., Tomar A., George S.P., Wang Y., Siddiqui M.R., Guo H., Tigyi G., Mathew S._x005F_x005F_x000D_Exp. Cell Res. 314:530-542(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF TYR-46; TYR-60; TYR-81; TYR-256; TYR-286; TYR-324; TYR-461; TYR-555; TYR-604 AND TYR-725, SUBCELLULAR LOCATION.Ref.20"A novel role for villin in intestinal epithelial cell survival and homeostasis."_x005F_x005F_x000D_Wang Y., Srinivasan K., Siddiqui M.R., George S.P., Tomar A., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 283:9454-9464(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION.Ref.21"Differential effects of lysophosphatidic acid and phosphatidylinositol 4,5-bisphosphate on actin dynamics by direct association with the actin-binding protein villin."_x005F_x005F_x000D_Tomar A., George S.P., Mathew S., Khurana S._x005F_x005F_x000D_J. Biol. Chem. 284:35278-35282(2009) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH LYSOPHOSPHATIDIC ACID, ASSOCIATION WITH F-ACTIN, SUBCELLULAR LOCATION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.057 ng/mL

Description: Description of target: Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.1;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 39 pg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF594 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF594 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), APC conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF680 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF680 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF680R conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), CF680R conjugate, Concentration: 0.1mg/mL